Project B01

Role of DEAD box helicases in kinase regulation during Wnt signaling

Principal investigator: Christof Niehrs


We have previously identified the DEAD-box RNA helicase DDX3 as a regulator of the Wnt/β-catenin network in embryonic development, where it acts as a regulatory subunit of Casein kinase 1 epsilon (CK1e). Our unpublished work indicates that other members of the 37 DEAD-box RNA helicases can also function as CK1 kinase regulators in Wnt signaling, and that this extends beyond CK1e. We therefore will validate if certain DEAD box helicases have a generic function in regulating diverse protein kinases during Wnt signaling. We aim to study the molecular interactions between four DDX helicases and three protein kinases and their physiological significance during Xenopus embryonic development.