Publikation-Schlagwort: NanoBRET

Research Article

Quantitative Profiling of WNT-3A Binding to All Human Frizzled Paralogues in HEK293 Cells by NanoBiT/BRET Assessments

Frizzled NanoBRET Wnt signaling

Kozielewicz P, Shekhani R, Moser S, Bowin CF, Wesslowski J, Davidson G, Schulte G. ACS Pharmacol Transl Sci. 2021 May 11;4(3):1235-1245. doi: 10.1021/acsptsci.1c00084. eCollection 2021 Jun 11.

PMID: 34151213

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Research Article

eGFP-tagged Wnt-3a enables functional analysis of Wnt trafficking and signaling and kinetic assessment of Wnt binding to full-length Frizzled

bioluminescence resonance energy transfer (BRET) Frizzled fusion protein G protein–coupled receptor (GPCR) ligand binding membrane protein NanoBiT NanoBRET trafficking Wnt signaling

Wesslowski J, Kozielewicz P, Wang X, Cui H, Schihada H, Kranz D, Karuna M P, Levkin P, Gross JC, Boutros M, Schulte G, Davidson G. J Biol Chem. 2020 May 7. pii: jbc.RA120.012892.

PMID:32381507

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