Research Article

NanoBiT- and NanoBiT/BRET-based assays allow the analysis of binding kinetics of WNT-3A to endogenous Frizzled 7 in a colorectal cancer model

binding affinity shift CRISPR/Cas9 FZD7 NanoBiT/BRET binding SW480 cells Wnt
Grätz L, Sajkowska-Kozielewicz JJ, Wesslowski J, Kinsolving J, Bridge LJ, Petzold K, Davidson G, Schulte G, Kozielewicz P. Br J Pharmacol. 2023 Apr 13. doi: 10.1111/bph.16090. Online ahead of print. PMID: 37055379
Learn more
Other

Tissue-Specific CRISPR-Cas9 Screening in Drosophila

CRISPR/Cas9 Drosophila Genome editing Screening sgRNA libraries
Port F, Boutros M. Methods Mol Biol. 2022;2540:157-176. doi: 10.1007/978-1-0716-2541-5_7. PMID: 35980577
Learn more
Research Article

Enhanced in vivo-imaging in medaka by optimized anaesthesia, fluorescent protein selection and removal of pigmentation

CRISPR/Cas9 in vivo-imaging zebrafish

Lischik CQ, Adelmann L, Wittbrodt J. PLoS One. 2019 Mar 7;14(3):e0212956.

PMID: 30845151

Learn more