A05

The Wnt code: Deciphering early Wnt interactions in Hydra

Funding period: 2017-2025

Project A05

Project Leaders

Prof. Dr. Thomas Holstein

Prof. Dr. Thomas Holstein

Project Leader A05
Prof. Dr. Motomu Tanaka

Prof. Dr. Motomu Tanaka

Project Leader A05

Abstract

Wnt genes are deeply embedded in metazoan genomes. It was shown that the complete repertoire of Wnt gene families known from vertebrates is present in cnidarians. Most Wnt genes are activated in a cascade-like manner (Wnt code) during pattern and axis formation. Now, the specific functions of different Wnt ligands will be dissected and their interplay with physical cues during regeneration will be analyzed in this collaborative project. It will provide a mechanistic understanding of how biophysical (mechanical) and biochemical (Wnt signaling) cues interact and how specific functions of various Wnt ligands were established in one of the most ancient signaling centers (Hydra head organizer) in animal evolution.

Project-related Publications

Two plus one is almost three: a fast approximation for multi-view deconvolution

Hüpfel M, Fernández Merino M, Bennemann J, Takamiya M, Rastegar S, Tursch A, Holstein TW, Nienhaus GU. Biomed Opt Express. 2021 Dec 7;13(1):147-158. doi: 10.1364/BOE.443660. eCollection 2022 Jan 1. PMID: 35154860

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The Wnt-specific astacin proteinase HAS-7 restricts head organizer formation in Hydra

Ziegler B, Yiallouros I, Trageser B, Kumar S, Mercker M, Kling S, Fath M, Warnken U, Schnölzer M, Holstein TW, Hartl M, Marciniak-Czochra A, Stetefeld J, Stöcker W, Özbek S. BMC Biol. 2021 Jun 9;19(1):120. 
PMID: 34107975

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Wnt/β-Catenin Signaling Controls Spatio-Temporal Elasticity Patterns in Extracellular Matrix during Hydra Morphogenesis

Veschgini, M., H.O. Petersen, F. Rossetti, W. Abuillan, S. Kaufmann, R. Suzuki, M. Burghammer, S. Özbek, T.W. Holstein, and M. Tanaka. 2020. bioRxiv, preprint.

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MAPK signaling links the injury response to Wnt-regulated patterning in Hydra regeneration

Tursch, A., M. Lommel, N. Bartsch, and T.W. Holstein. 2020.  bioRxiv, preprint.

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Expansion of a single transposable element family is associated with genome-size increase and radiation in the genus Hydra

Wong WY, Simakov O, Bridge DM, Cartwright P, Bellantuono AJ, Kuhn A, Holstein TW, David CN, Steele RE, Martínez DE. Proc Natl Acad Sci U S A. 2019 Nov 12;116(46):22915-22917. PMID: 31659034

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What makes flatworms go to pieces

Holstein TW. Nature. 2019 Aug;572(7771):593-594. PMID: 31444489

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Hydra Mesoglea Proteome Identifies Thrombospondin as a Conserved Component Active in Head Organizer Restriction

Lommel M, Strompen J, Hellewell AL, Balasubramanian GP, Christofidou ED, Thomson AR, Boyle AL, Woolfson DN, Puglisi K, Hartl M, Holstein TW, Adams JC, Özbek S. Sci Rep. 2018 Aug 6;8(1):11753. PMID:30082916

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Making head or tail of cnidarian hox gene function

Rentzsch F, Holstein TW. Nat Commun. 2018 Jun 5;9(1):2187. PMID:29872045

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Tracking mechanical and morphological dynamics of regenerating Hydra tissue fragments using a two fingered micro-robotic hand

Veschgini, M., Gebert, F., Khangai, N., Ito, H., Suzuki, R., Holstein, T. W., Mae, Y., Arai, T. and Tanaka, M. 2016. Appl. Phys. Lett. 108, 103702

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A Comprehensive Transcriptomic and Proteomic Analysis of Hydra Head Regeneration.

Petersen, H.O., S.K. Hoger, M. Looso, T. Lengfeld, A. Kuhn, U. Warnken, C. Nishimiya-Fujisawa, M. Schnolzer, M. Kruger, S. Ozbek, O. Simakov, and T.W. Holstein. Mol Biol Evol. 2015 Aug;32(8):1928-47. doi: 10.1093/molbev/msv079. Epub 2015 Apr 3. PMID: 25841488

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Nodal signalling determines biradial asymmetry in Hydra

Watanabe H, Schmidt HA, Kuhn A, Höger SK, Kocagöz Y, Laumann-Lipp N, Ozbek S, Holstein TW. Nature. 2014 Nov 6;515(7525):112-5. doi: 10.1038/nature13666. Epub 2014 Aug 24.
PMID: 25156256

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Autoregulatory and repressive inputs localize Hydra Wnt3 to the head organizer

Nakamura Y, Tsiairis CD, Özbek S, Holstein TW. Proc Natl Acad Sci U S A. 2011 May 31;108(22):9137-42. doi: 10.1073/pnas.1018109108. Epub 2011 May 16. PMID: 21576458

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Unexpected complexity of the Wnt gene family in a sea anemone

Kusserow A, Pang K, Sturm C, Hrouda M, Lentfer J, Schmidt HA, Technau U, von Haeseler A, Hobmayer B, Martindale MQ, Holstein TW. Nature. 2005 Jan 13;433(7022):156-60. doi: 10.1038/nature03158.
PMID: 15650739

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WNT signalling molecules act in axis formation in the diploblastic metazoan Hydra

Hobmayer B, Rentzsch F, Kuhn K, Happel CM, von Laue CC, Snyder P, Rothbächer U, Holstein TW. Nature. 2000 Sep 14;407(6801):186-9. doi: 10.1038/35025063.
PMID: 11001056

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