Prof. Dr. Michael Boutros

Project Leader A01, A04, Z01, Z03

Heidelberg University
Department of Cell and Molecular biology / MEDMA & DKFZ
BioQuant - Center for Quantitative Analysis of Molecular and Cellular Biosystems
Im Neuenheimer Feld 267
D-69120 Heidelberg

Prof. Dr. Michael Boutros

Professional Career

since 2020


Marsilius Kolleg, Heidelberg University

since 2008

Professor for Cell- and Molecular Biology

Medical Faculty Mannheim, Heidelberg University, Heidelberg

since 2008

Head of Division

Signaling and Functional Genomics, German Cancer Research Center, Heidelberg


Group leader

Emmy Noether Program of the DFG, German Cancer Research Center, Heidelberg


Postdoctoral research fellow

Norbert Perrimon, Department of Genetics, Harvard Medical School, Boston, USA



Kennedy School of Government, Harvard University, Boston, USA


Dr. rer. nat.

European Molecular Biology Laboratory and Heidelberg University; Advisor: Prof. Marek Mlodzik

Achievements & Awards

since 2021


DFG Collaborative Research Center 1324

since 2020


Research and Strategy Commission, Heidelberg University

since 2019

Coordinating PI

ERC Synergy Grant DECODE



DFG Collaborative Research Center 1324

since 2016

Elected Member

DFG panel 201-02 ‘Genetics’


Scientific Director (interim)

German Cancer Research Center, Heidelberg

since 2014

Editorial Board Member

Molecular Systems Biology


Elected member

Senate of Heidelberg University


EMBO Member

European Molecular Biology Organization


ERC Advanced Grant

European Research Council

since 2012

Member of the Executive Board and PI

Excellence Cluster CellNetworks, Heidelberg

since 2012

University Member of the Board (Kuratorium) and since 2014 Deputy Chair of Board

Studienstiftung des deutschen Volkes


Vice Dean for Research

Medical Faculty Mannheim, Heidelberg University

since 2008

Member of the Executive Board and PI

HBIGS (Excellence Initiative), Heidelberg University


Johann-Georg Zimmermann Award for Cancer Research

Funded by the German Hypothekenbank AG in cooperation with the Hannover Medical School


EMBO Young Investigator Award

European Molecular Biology Organization



Die Junge Akademie at the Berlin-Brandenburgischen Academy of Science and the Academy Leopoldina


Emmy-Noether Postdoctoral Fellowship and Junior Group



Ph.D. fellowship

Boehringer Ingelheim



Studienstiftung des deutschen Volkes


A temperature-tolerant CRISPR base editor mediates highly efficient and precise gene editing in Drosophila

Doll RM, Boutros M, Port F. Sci Adv. 2023 Sep;9(35):eadj1568. doi: 10.1126/sciadv.adj1568. Epub 2023 Aug 30. PMID: 37647411

Learn more
Glyoxal as alternative fixative for single cell RNA sequencing

Bageritz J, Krausse N, Yousefian S, Leible S, Valentini E, Boutros M. G3 (Bethesda). 2023 Jul 26:jkad160. doi: 10.1093/g3journal/jkad160. Online ahead of print. PMID: 37494060

Learn more
The Roles of Secreted Wnt Ligands in Cancer

Werner J, Boonekamp KE, Zhan T, Boutros M. Int J Mol Sci. 2023 Mar 10;24(6):5349. doi: 10.3390/ijms24065349. PMID: 36982422

Learn more
The role of Evi/Wntless in exporting Wnt proteins

Wolf L, Boutros M. Development. 2023 Feb 15;150(3):dev201352. doi: 10.1242/dev.201352. Epub 2023 Feb 10.
PMID: 36763105

Learn more
Blockade of Wnt Secretion Attenuates Myocardial Ischemia-Reperfusion Injury by Modulating the Inflammatory Response

Meyer IS, Li X, Meyer C, Voloshanenko O, Pohl S, Boutros M, Katus HA, Frey N, Leuschner F. Int J Mol Sci. 2022 Oct 14;23(20):12252. doi: 10.3390/ijms232012252.
PMID: 36293109

Learn more
Tissue-Specific CRISPR-Cas9 Screening in Drosophila

Port F, Boutros M. Methods Mol Biol. 2022;2540:157-176. doi: 10.1007/978-1-0716-2541-5_7. PMID: 35980577

Learn more
Superresolution microscopy localizes endogenous Dvl2 to Wnt signaling-responsive biomolecular condensates

Schubert A, Voloshanenko O, Ragaller F, Gmach P, Kranz D, Scheeder C, Miersch T, Schulz M, Trümper L, Binder C, Lampe M, Engel U, Boutros M. Proc Natl Acad Sci U S A. 2022 Jul 26;119(30):e2122476119. doi: 10.1073/pnas.2122476119. Epub 2022 Jul 22. PMID: 35867833

Learn more
Allele-specific endogenous tagging and quantitative analysis of β-catenin in colorectal cancer cells

Ambrosi G, Voloshanenko O, Eckert AF, Kranz D, Nienhaus GU, Boutros M. Elife. 2022 Jan 11;11:e64498. doi: 10.7554/eLife.64498.
PMID: 35014953

Learn more
EVI/WLS function is regulated by ubiquitination and linked to ER-associated degradation by ERLIN2

Wolf LM, Lambert AM, Haenlin J, Boutros M. J Cell Sci. 2021 Jul 19:jcs.257790. doi: 10.1242/jcs.257790. Online ahead of print.
PMID: 3427964

Learn more
A spatial vascular transcriptomic, proteomic, and phosphoproteomic atlas unveils an angiocrine Tie–Wnt signaling axis in the liver

Inverso D, Shi J, Lee KH, Jakab M, Ben-Moshe S, Kulkarni SR, Schneider M, Wang G, Komeili M, Vélez PA, Riedel M, Spegg C, Ruppert T, Schaeffer-Reiss C, Helm D, Singh I, Boutros M, Chintharlapalli S, Heikenwalder M, Itzkovitz S, Augustin HG. Dev Cell. 2021 Jun 7;56(11):1677-1693.e10. PMID: 34038707

Learn more
Multi-omics integration identifies a selective vulnerability of colorectal cancer subtypes to YM155

Zhan T, Faehling V, Rauscher B, Betge J, Ebert MP, Boutros M. Int J Cancer. 2021 Apr 15;148(8):1948-1963. doi: 10.1002/ijc.33393. Epub 2020 Dec 4.
PMID: 33186476

Learn more
Wnt10b GSK3β dependent Wnt/STOP signaling prevents aneuploidy in human somatic cells

Lin YC, Haas A, Bufe A, Parbin S, Hennecke M, Voloshanenko O, Gross J, Boutros M, Acebron SP, Bastians H. Life Sci Alliance. 2020 Nov 30;4(1):e202000855. Print 2021 Jan.
PMID: 33257473

Learn more
Extracellular vesicles and oncogenic signaling

Schubert A, Boutros M. Mol Oncol. 2020 Nov 18. doi: 10.1002/1878-0261.12855. PMID: 33207034

Learn more
Multiplexed conditional genome editing with Cas12a in Drosophila

Port F, Starostecka M, Boutros M. Proc Natl Acad Sci U S A. 2020 Aug 25:202004655.

PMID: 32843348

Learn more
eGFP-tagged Wnt-3a enables functional analysis of Wnt trafficking and signaling and kinetic assessment of Wnt binding to full-length Frizzled

Wesslowski J, Kozielewicz P, Wang X, Cui H, Schihada H, Kranz D, Karuna M P, Levkin P, Gross JC, Boutros M, Schulte G, Davidson G. J Biol Chem. 2020 May 7. pii: jbc.RA120.012892. PMID:32381507

Learn more
gscreend: modelling asymmetric count ratios in CRISPR screens to decrease experiment size and improve phenotype detection

Imkeller K, Ambrosi G, Boutros M, Huber W. Genome Biol. 2020 Mar 2; 21(1):53. PMID: 32122365

Learn more
A Large-Scale Resource for Tissue-Specific CRISPR Mutagenesis in Drosophila

Port F, Strein C, Stricker M, Rauscher B, Heigwer F, Zhou J, Beyersdörffer C, Frei J, Hess A, Kern K, Lange L, Langner N, Malamud R, Pavlović B, Rädecke K, Schmitt L, Voos L, Valentini E, Boutros M. Elife. 2020 Feb 13;9:e53865.

PMID: 32053108

Learn more
Exocyst-mediated apical Wg secretion activates signaling in the Drosophila wing epithelium

Chaudhary V, Boutros M. PLoS Genet. 2019 Sep 17;15(9):e1008351. PMID: 31527874

Learn more
Robust Wnt signaling is maintained by a Wg protein gradient and Fz2 receptor activity in the developing Drosophila wing

Chaudhary V, Hingole S, Frei J, Port F, Strutt D, Boutros M. Development. 2019 Aug 9;146(15). PMID: 31399474

Learn more
Gene expression atlas of a developing tissue by single cell expression correlation analysis

Bageritz J, Willnow P, Valentini E, Leible S, Boutros M, Teleman AA. Nat Methods. 2019 Aug;16(8):750-756. doi: 10.1038/s41592-019-0492-x. Epub 2019 Jul 29. PMID: 31363221

Learn more
MEK inhibitors activate Wnt signalling and induce stem cell plasticity in colorectal cancer

Zhan T, Ambrosi G, Wandmacher AM, Rauscher B, Betge J, Rindtorff N, Häussler RS, Hinsenkamp I, Bamberg L, Hessling B, Müller-Decker K, Erdmann G, Burgermeister E, Ebert MP, Boutros M. Nat Commun. 2019 May 16;10(1):2197. doi: 10.1038/s41467-019-09898-0. PMID: 31097693

Learn more
WEADE: a workflow for enrichment analysis and data exploration

Trost N, Rempel E, Ermakova O, Tamirisa S, Pârcălăbescu L, Boutros M, Lohmann JU, Lohmann I. PLoS One. 2018 Sep 28;13(9):e0204016. doi: 10.1371/journal.pone.0204016. eCollection 2018. PMID: 30265728

Learn more
Decoding the regulatory logic of the Drosophila male stem cell system

Tamirisa S, Papagiannouli F, Rempel E, Ermakova O, Trost N, Zhou J, Mundorf J, Brunel S, Ruhland N, Boutros M, Lohmann JU, Lohmann I. Cell Rep. 2018 Sep 11;24(11):3072-3086. doi: 10.1016/j.celrep.2018.08.013. PMID: 30208329

Learn more
Toward an integrated map of genetic interactions in cancer cells

Rauscher B, Heigwer F, Henkel L, Hielscher T, Voloshanenko O, Boutros M. Mol Syst Biol. 2018 Feb 21;14(2):e7656. doi: 10.15252/msb.20177656. PMID: 29467179

Learn more
β-catenin-independent regulation of Wnt target genes by RoR2 and ATF2/ATF4 in colon cancer cells

Voloshanenko O, Schwartz U, Kranz D, Rauscher B, Linnebacher M, Augustin I, Boutros M. Sci Rep. 2018 Feb 16;8(1):3178. PMID:29453334

Learn more
ERAD-dependent control of the Wnt secretory factor Evi

Glaeser K, Urban M, Fenech E, Voloshanenko O, Kranz D, Lari F, Christianson JC, Boutros M. EMBO J. 2018 Feb 15;37(4). PMID:29378775  

Learn more
The cardiac microenvironment uses non-canonical WNT signaling to activate monocytes after myocardial infarction

Meyer IS, Jungmann A, Dieterich C, Zhang M, Lasitschka F, Werkmeister S, Haas J, Müller OJ, Boutros M, Nahrendorf M, Katus HA, Hardt SE, Leuschner F. EMBO Mol Med. 2017 Sep;9(9):1279-1293. doi: 10.15252/emmm.201707565. PMID: 28774883

Learn more
Mapping of Wnt-Frizzled interactions by multiplex CRISPR targeting of receptor gene families

Voloshanenko O, Gmach P, Winter J, Kranz D, Boutros M. FASEB J. 2017 Nov;31(11):4832-4844. doi: 10.1096/fj.201700144R. Epub 2017 Jul 21. PMID: 28733458

Learn more
Molecular dissection of Wnt3a-Frizzled8 interaction reveals essential and modulatory determinants of Wnt signaling activity

Kumar S, Žigman M, Patel TR, Trageser B, Gross JC, Rahm K, Boutros M, Gradl D, Steinbeisser H, Holstein T, Stetefeld J, Özbek S. BMC Biol. 2014 May 30;12:44. doi: 10.1186/1741-7007-12-44. PMID: 24885675

Learn more
The microtubule affinity regulating kinase MARK4 promotes axoneme extension during early ciliogenesis

Kuhns S, Schmidt KN, Reymann J, Gilbert DF, Neuner A, Hub B, Carvalho R, Wiedemann P, Zentgraf H, Erfle H, Klingmüller U, Boutros M, Pereira G. J Cell Biol. 2013 Feb 18;200(4):505-22. doi: 10.1083/jcb.201206013. Epub 2013 Feb 11. PMID: 23400999

Learn more
Active Wnt proteins are secreted on exosomes

Gross JC, Chaudhary V, Bartscherer K, Boutros M. Nat Cell Biol. 2012 Oct;14(10):1036-45. doi: 10.1038/ncb2574. Epub 2012 Sep 16. PMID: 22983114

Learn more
Cell cycle control of wnt receptor activation

Davidson G, Shen J, Huang YL, Su Y, Karaulanov E, Bartscherer K, Hassler C, Stannek P, Boutros M, Niehrs C. Dev Cell. 2009 Dec;17(6):788-99. doi: 10.1016/j.devcel.2009.11.006. PMID: 20059949

Learn more
Secretion of Wnt ligands requires Evi, a conserved transmembrane protein

Bartscherer K, Pelte N, Ingelfinger D, Boutros M. Cell. 2006 May 5;125(3):523-33. doi: 10.1016/j.cell.2006.04.009. PMID: 16678096

Learn more