A04

Roles of lipids in Wnt secretion and signaling

Funding period: 2017-2025

Project A05

Project Leaders

Prof. Dr. Britta Brügger

Prof. Dr. Britta Brügger

 Project Leader A04
Prof. Dr. Michael Boutros

Prof. Dr. Michael Boutros

 Project Leader A01, A04, Z01, Z03

Abstract

Lipids play essential roles in Wnt function, including the covalent attachment of fatty acids to Wnt proteins and as membrane domains in which Wnt-receptor complexes reside to initiate Wnt signaling. Deciphering how covalently attached fatty acids and membrane lipids that surround proteins of the Wnt signaling complex govern Wnt activity will provide fundamental insight into Wnt biology. By combining the expertise in lipid biology from the Brügger group and Wnt biology from the Boutros group, we aim to address these questions by utilizing chemical biology, biochemical reconstitution experiments and cell-based analyses to uncover novel principles to regulate Wnt functions.

Project-related Publications

Cholesterol Localization around the Metabotropic Glutamate Receptor 2

Kurth M, Lolicato F, Sandoval-Perez A, Amaya-Espinosa H, Teslenko A, Sinning I, Beck R, Brügger B, Aponte-Santamaría C. J Phys Chem B. 2020 Oct 15;124(41):9061-9078. doi: 10.1021/acs.jpcb.0c05264. Epub 2020 Oct 2.
PMID: 32954729

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Protease‐resistant streptavidin for interaction proteomics

Rafiee MR, Sigismondo G, Kalxdorf M, Förster L, Brügger B, Béthune J, Krijgsveld J. Mol Syst Biol. 2020 May;16(5):e9370. PMID: 32400114

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CerS6-Derived Sphingolipids Interact with Mff and Promote Mitochondrial Fragmentation in Obesity

Hammerschmidt P, Ostkotte D, Nolte H, Gerl MJ, Jais A, Brunner HL, Sprenger HG, Awazawa M, Nicholls HT, Turpin-Nolan SM, Langer T, Krüger M, Brügger B, Brüning JC. Cell. 2019 May 30;177(6):1536-1552.e23. doi: 10.1016/j.cell.2019.05.008.
PMID: 31150623

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A method for the quantitative determination of glycerophospholipid regioisomers by UPLC-ESI-MS/MS

Wozny K, Lehmann WD, Wozny M, Akbulut BS, Brügger B. Anal Bioanal Chem. 2019 Feb;411(4):915-924. doi: 10.1007/s00216-018-1517-5. Epub 2018 Dec 22. PMID: 30580388

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β-catenin-independent regulation of Wnt target genes by RoR2 and ATF2/ATF4 in colon cancer cells

Voloshanenko O, Schwartz U, Kranz D, Rauscher B, Linnebacher M, Augustin I, Boutros M. Sci Rep. 2018 Feb 16;8(1):3178. PMID:29453334

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Mapping of Wnt-Frizzled interactions by multiplex CRISPR targeting of receptor gene families

Voloshanenko O, Gmach P, Winter J, Kranz D, Boutros M. FASEB J. 2017 Nov;31(11):4832-4844. doi: 10.1096/fj.201700144R. Epub 2017 Jul 21. PMID: 28733458

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Sphingosine-1-phosphate lyase deficient cells as a tool to study protein lipid interactions

Gerl MJ, Bittl V, Kirchner S, Sachsenheimer T, Brunner HL, Lüchtenborg C, Özbalci C, Wiedemann H, Wegehingel S, Nickel W, Haberkant P, Schultz C, Krüger M, Brügger B. PLoS One. 2016 Apr 21;11(4):e0153009. doi: 10.1371/journal.pone.0153009. eCollection 2016. PMID: 27100999

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Analysis of transmembrane domains and lipid modified peptides with matrix-assisted laser desorption ionizationtime-of-flight mass spectrometry

Gerl MJ, Sachsenheimer T, Grzybek M, Coskun U, Wieland FT, Brügger B. Anal Chem. 2014 Apr 15;86(8):3722-6. doi: 10.1021/ac500446z. Epub 2014 Mar 24.
PMID: 24628620

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