A08

Wnt pathway specificity in niche-stem cell control

Funding period: 2021-2025

Project A08

Project Leaders

Dr. Josephine Bageritz

Dr. Josephine Bageritz

 Project Leader A08
Prof. Dr. Ingrid Lohmann

Prof. Dr. Ingrid Lohmann

 Project Leader A08

Abstract

Wnt signaling is critical for stem cell development and homeostasis. However, it is still underexplored how Wnt pathways control different stem cell populations within one tissue in a cell-type specific manner. We will study two stem cell niches in Drosophila to elucidate context-dependent Wnt input and output codes influenced by cross-talk with other signaling pathways at the single cell level. Comparison and functional analysis of the Wnt code will be instrumental in understanding the basic principles as well as the particularities of Wnt encoded specificity in different stem cell types.

Project-related Publications

Analysis of Single-Cell Transcriptome Data in Drosophila

Yousefian S, Musillo MJ, Bageritz J. Methods Mol Biol. 2022;2540:93-111. doi: 10.1007/978-1-0716-2541-5_4. PMID: 35980574

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The molecular logic of synaptic wiring at the single cell level

Velten, J., R. Agarwal, P. van Nierop y Sanchez, L. Bognar, M. Paulsen, L. Velten, and I. Lohmann. 2021. bioRxiv, preprint.

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Closing the gap: a roadmap to single-cell regulatory genomics

Carnesecchi J, Lohmann I. Mol Syst Biol. 2020 May;16(5):e9497. doi: 10.15252/msb.20209497. PMID: 32430985

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ATF4-induced Warburg Metabolism drives Over-proliferation in Drosophila

Sorge S, Theelke J, Yildirim K, Hertenstein H, McMullen E, Müller S, Altbürger C, Schirmeier S, Lohmann I. Cell Rep. 2020 May 19;31(7):107659. doi: 10.1016/j.celrep.2020.107659. PMID: 32433968

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Multi-level and lineage-specific interactomes of the Hox transcription factor Ubx contribute to its functional specificity

Carnesecchi J, Sigismondo G, Domsch K, Baader CEP, Rafiee MR, Krijgsveld J, Lohmann I. Nat Commun. 2020 Mar 13;11(1):1388. doi: 10.1038/s41467-020-15223-x. PMID: 32170121

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Gene expression atlas of a developing tissue by single cell expression correlation analysis

Bageritz J, Willnow P, Valentini E, Leible S, Boutros M, Teleman AA. Nat Methods. 2019 Aug;16(8):750-756. doi: 10.1038/s41592-019-0492-x. Epub 2019 Jul 29. PMID: 31363221

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Single-Cell RNA Sequencing with Drop-Seq

Bageritz J, Raddi G. Methods Mol Biol. 2019;1979:73-85. doi: 10.1007/978-1-4939-9240-9_6.
PMID: 31028633

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WEADE: a workflow for enrichment analysis and data exploration

Trost N, Rempel E, Ermakova O, Tamirisa S, Pârcălăbescu L, Boutros M, Lohmann JU, Lohmann I. PLoS One. 2018 Sep 28;13(9):e0204016. doi: 10.1371/journal.pone.0204016. eCollection 2018. PMID: 30265728

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Decoding the regulatory logic of the Drosophila male stem cell system

Tamirisa S, Papagiannouli F, Rempel E, Ermakova O, Trost N, Zhou J, Mundorf J, Brunel S, Ruhland N, Boutros M, Lohmann JU, Lohmann I. Cell Rep. 2018 Sep 11;24(11):3072-3086. doi: 10.1016/j.celrep.2018.08.013. PMID: 30208329

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Stk33 is required for spermatid differentiation and male fertility in mice

Martins LR, Bung RK, Koch S, Richter K, Schwarzmüller L, Terhardt D, Kurtulmus B, Niehrs C, Rouhi A, Lohmann I, Pereira G, Fröhling S, Glimm H, Scholl C. Dev Biol. 2018 Jan 1;433(1):84-93. doi: 10.1016/j.ydbio.2017.11.007. Epub 2017 Nov 16. PMID: 29155043

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The Hox gene Abd-B controls stem cell niche function in the Drosophila testis

Papagiannouli F, Schardt L, Grajcarek J, Ha N, Lohmann I. Dev Cell. 2014 Jan 27;28(2):189-202. doi: 10.1016/j.devcel.2013.12.016. PMID: 24480643

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