B02

Mechanism of oscillatory Wnt signaling dynamics and its role in vertebrate embryos

Funding period: 2017-2025

Project B02

Project Leaders

Dr. Alexander Aulehla

Dr. Alexander Aulehla

Project Leader B02

Abstract

The mechanism underlying oscillatory Wnt signaling dynamics and its link to metabolic activity during vertebrate embryonic development will be addressed in the context of the embryonic somite segmentation clock. To this end, a quantitative approach that combines real-time imaging of customized Wnt reporter knock-in lines, new genetic animal models and time-resolved functional perturbations will be used. The project is based on established in vitro and in vivo segmentation clock models, including the mouse and medaka. A particular aim is to reveal core mechanistic principles underlying Wnt signaling dynamics and its link to metabolic pathways, enabling comparison to other developmental disease models.

Project-related Publications

Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development

Bulusu V, Prior N, Snaebjornsson MT, Kuehne A, Sonnen KF, Kress J, Stein F, Schultz C, Sauer U, Aulehla A. Dev Cell. 2017 Feb 27;40(4):331-341.e4. doi: 10.1016/j.devcel.2017.01.015. PMID: 28245920

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Self-Organization of Embryonic Genetic Oscillators into Spatiotemporal Wave Patterns

Tsiairis CD, Aulehla A. Cell. 2016 Feb 11;164(4):656-67. doi: 10.1016/j.cell.2016.01.028. PMID: 26871631

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Modulation of Phase Shift between Wnt and Notch Signaling Oscillations Controls Mesoderm Segmentation

Tsiairis CD, Aulehla A. Cell. 2016 Feb 11;164(4):656-67. doi: 10.1016/j.cell.2016.01.028. PMID: 26871631

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Scaling of embryonic patterning based on phase-gradient encoding

Lauschke VM, Tsiairis CD, François P, Aulehla A. Nature. 2013 Jan 3;493(7430):101-5. doi: 10.1038/nature11804. Epub 2012 Dec 19.
PMID: 23254931

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