Z02

Advanced fluorescence microscopy

Funding period: 2017-2025

Project Z02

Project Leaders

Prof. Dr. Gerd Ulrich Nienhaus

Prof. Dr. Gerd Ulrich Nienhaus

Project Leader A06, Z02
Dr. Ulrike Engel

Dr. Ulrike Engel

Project Leader Z02

Abstract

Central project Z02 provides complementary expertise at its locations in Heidelberg and Karlsruhe and access to a wide range of advanced fluorescence imaging techniques. Besides conventional laser scanning confocal microscopy (CSLM) and widefield microscopy, we employ super-resolution methods such as STED microscopy, structured illumination microscopy (SIM) and single-molecule localization microscopy (SMLM) including a powerful digital light sheet microscope (DSLM) for fast 3D imaging built in the previous funding period. With these powerful imaging tools, we will image and quantitatively analyze processes associated with Wnt signaling in cells, tissues and entire model organisms in all three spatial dimensions and in time.

Project-related Publications

Allele-specific endogenous tagging and quantitative analysis of β-catenin in colorectal cancer cells

Ambrosi G, Voloshanenko O, Eckert AF, Kranz D, Nienhaus GU, Boutros M. Elife. 2022 Jan 11;11:e64498. doi: 10.7554/eLife.64498.
PMID: 35014953

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Two plus one is almost three: a fast approximation for multi-view deconvolution

Hüpfel M, Fernández Merino M, Bennemann J, Takamiya M, Rastegar S, Tursch A, Holstein TW, Nienhaus GU. Biomed Opt Express. 2021 Dec 7;13(1):147-158. doi: 10.1364/BOE.443660. eCollection 2022 Jan 1. PMID: 35154860

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Wnt signaling recruits KIF2A to the spindle to ensure chromosome congression and alignment during mitosis

Bufe A, García Del Arco A, Hennecke M, de Jaime-Soguero A, Ostermaier M, Lin YC, Ciprianidis A, Hattemer J, Engel U, Beli P, Bastians H, Acebrón SP. Proc Natl Acad Sci U S A. 2021 Aug 24; 118(34):e2108145118. doi:10.1073/pnas.2108145118. PMID: 34417301

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Measuring ligand-cell surface receptor affinities with axial line-scanning fluorescence correlation spectroscopy

Eckert AF, Gao P, Wesslowski J, Wang X, Rath J, Nienhaus K, Davidson G, Nienhaus GU. Elife. 2020 May 22;9:e55286.

PMID: 32441251

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Wnt/PCP controls spreading of Wnt/β-catenin signals by cytonemes in vertebrates

Mattes B, Dang Y, Greicius G, Kaufmann LT, Prunsche B, Rosenbauer J, Stegmaier J, Mikut R, Özbek S, Nienhaus GU, Schug A, Virshup DM, Scholpp S. Elife. 2018 Jul 31;7. PMID:30060804

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Characterization of spindle pole body duplication reveals a regulatory role for nuclear pore complexes

Rüthnick D, Neuner A, Dietrich F, Kirrmaier D, Engel U, Knop M, Schiebel E. J Cell Biol. 2017 Aug 7;216(8):2425-2442. doi: 10.1083/jcb.201612129. Epub 2017 Jun 28. PMID: 28659328

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Four-Channel Super-Resolution Imaging by 3-D Structured Illumination

Engel U. Methods Mol Biol. 2017;1663:79-94. doi: 10.1007/978-1-4939-7265-4_7.
PMID: 28924660

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Early Commissural Diencephalic Neurons Control Habenular Axon Extension and Targeting

Beretta CA, Dross N, Guglielmi L, Bankhead P, Soulika M, Gutierrez-Triana JA, Paolini A, Poggi L, Falk J, Ryu S, Kapsimali M, Engel U, Carl M. Curr Biol. 2017 Jan 23;27(2):270-278. doi: 10.1016/j.cub.2016.11.038. Epub 2017 Jan 5. PMID: 28065605

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