Z02

Advanced fluorescence microscopy

Funding period: 2017-2025

Project Leaders

Prof. Dr. Gerd Ulrich Nienhaus

Project Leader A06, Z02

Dr. Ulrike Engel

Project Leader Z02

Abstract

Central project Z02 provides complementary expertise at its locations in Heidelberg and Karlsruhe and access to a wide range of advanced fluorescence imaging techniques. Besides conventional laser scanning confocal microscopy (CSLM) and widefield microscopy, we employ super-resolution methods such as STED microscopy, structured illumination microscopy (SIM) and single-molecule localization microscopy (SMLM) including a powerful digital light sheet microscope (DSLM) for fast 3D imaging built in the previous funding period. With these powerful imaging tools, we will image and quantitatively analyze processes associated with Wnt signaling in cells, tissues and entire model organisms in all three spatial dimensions and in time.

Project-related Publications

Wnt/β-catenin signaling induces axial elasticity patterns of Hydra extracellular matrix

Veschgini M, Suzuki R, Kling S, Petersen HO, Bergheim BG, Abuillan W, Linke P, Kaufmann S, Burghammer M, Engel U, Stein F, Özbek S, Holstein TW, Tanaka M. iScience. 2023 Mar 16;26(4):106416. doi: 10.1016/j.isci.2023.106416. eCollection 2023 Apr 21. PMID: 37009232

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Superresolution microscopy localizes endogenous Dvl2 to Wnt signaling-responsive biomolecular condensates

Schubert A, Voloshanenko O, Ragaller F, Gmach P, Kranz D, Scheeder C, Miersch T, Schulz M, Trümper L, Binder C, Lampe M, Engel U, Boutros M. Proc Natl Acad Sci U S A. 2022 Jul 26;119(30):e2122476119. doi: 10.1073/pnas.2122476119. Epub 2022 Jul 22. PMID: 35867833

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Genetically encodable fluorescent protein markers in advanced optical imaging

Nienhaus K, Nienhaus GU. Methods Appl Fluoresc. 2022 Jun 29. doi: 10.1088/2050-6120/ac7d3f. Online ahead of print.

PMID: 35767981

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Allele-specific endogenous tagging and quantitative analysis of β-catenin in colorectal cancer cells

Ambrosi G, Voloshanenko O, Eckert AF, Kranz D, Nienhaus GU, Boutros M. Elife. 2022 Jan 11;11:e64498. doi: 10.7554/eLife.64498.

PMID: 35014953

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Two plus one is almost three: a fast approximation for multi-view deconvolution

Hüpfel M, Fernández Merino M, Bennemann J, Takamiya M, Rastegar S, Tursch A, Holstein TW, Nienhaus GU. Biomed Opt Express. 2021 Dec 7;13(1):147-158. doi: 10.1364/BOE.443660. eCollection 2022 Jan 1. PMID: 35154860

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Wnt signaling recruits KIF2A to the spindle to ensure chromosome congression and alignment during mitosis

Bufe A, García Del Arco A, Hennecke M, de Jaime-Soguero A, Ostermaier M, Lin YC, Ciprianidis A, Hattemer J, Engel U, Beli P, Bastians H, Acebrón SP. Proc Natl Acad Sci U S A. 2021 Aug 24; 118(34):e2108145118. doi:10.1073/pnas.2108145118. PMID: 34417301

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Measuring ligand-cell surface receptor affinities with axial line-scanning fluorescence correlation spectroscopy

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Wnt/PCP controls spreading of Wnt/β-catenin signals by cytonemes in vertebrates

Mattes B, Dang Y, Greicius G, Kaufmann LT, Prunsche B, Rosenbauer J, Stegmaier J, Mikut R, Özbek S, Nienhaus GU, Schug A, Virshup DM, Scholpp S. Elife. 2018 Jul 31;7. PMID:30060804

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Characterization of spindle pole body duplication reveals a regulatory role for nuclear pore complexes

Rüthnick D, Neuner A, Dietrich F, Kirrmaier D, Engel U, Knop M, Schiebel E. J Cell Biol. 2017 Aug 7;216(8):2425-2442. doi: 10.1083/jcb.201612129. Epub 2017 Jun 28. PMID: 28659328

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Four-Channel Super-Resolution Imaging by 3-D Structured Illumination

Engel U. Methods Mol Biol. 2017;1663:79-94. doi: 10.1007/978-1-4939-7265-4_7.

PMID: 28924660

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Early Commissural Diencephalic Neurons Control Habenular Axon Extension and Targeting

Beretta CA, Dross N, Guglielmi L, Bankhead P, Soulika M, Gutierrez-Triana JA, Paolini A, Poggi L, Falk J, Ryu S, Kapsimali M, Engel U, Carl M. Curr Biol. 2017 Jan 23;27(2):270-278. doi: 10.1016/j.cub.2016.11.038. Epub 2017 Jan 5. PMID: 28065605

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