Advanced fluorescence microscopy
Funding period: 2017-2025

Project Leaders
Abstract
Central project Z02 provides complementary expertise at its locations in Heidelberg and Karlsruhe and access to a wide range of advanced fluorescence imaging techniques. Besides conventional laser scanning confocal microscopy (CSLM) and widefield microscopy, we employ super-resolution methods such as STED microscopy, structured illumination microscopy (SIM) and single-molecule localization microscopy (SMLM) including a powerful digital light sheet microscope (DSLM) for fast 3D imaging built in the previous funding period. With these powerful imaging tools, we will image and quantitatively analyze processes associated with Wnt signaling in cells, tissues and entire model organisms in all three spatial dimensions and in time.

Project-related Publications
Wnt/β-catenin signaling induces axial elasticity patterns of Hydra extracellular matrix
Superresolution microscopy localizes endogenous Dvl2 to Wnt signaling-responsive biomolecular condensates
Proc Natl Acad Sci U S A. 2022 Jul 26;119(30):e2122476119. doi: 10.1073/pnas.2122476119. Epub 2022 Jul 22. PMID: 35867833
Learn moreGenetically encodable fluorescent protein markers in advanced optical imaging
Allele-specific endogenous tagging and quantitative analysis of β-catenin in colorectal cancer cells
Two plus one is almost three: a fast approximation for multi-view deconvolution
Biomed Opt Express. 2021 Dec 7;13(1):147-158. doi: 10.1364/BOE.443660. eCollection 2022 Jan 1. PMID: 35154860
Learn moreWnt signaling recruits KIF2A to the spindle to ensure chromosome congression and alignment during mitosis
Measuring ligand-cell surface receptor affinities with axial line-scanning fluorescence correlation spectroscopy
Wnt/PCP controls spreading of Wnt/β-catenin signals by cytonemes in vertebrates
Mattes B, Dang Y, Greicius G, Kaufmann LT, Prunsche B, Rosenbauer J, Stegmaier J, Mikut R, Özbek S, Nienhaus GU, Schug A, Virshup DM, Scholpp S. Elife. 2018 Jul 31;7. PMID:30060804
Learn moreCharacterization of spindle pole body duplication reveals a regulatory role for nuclear pore complexes
J Cell Biol. 2017 Aug 7;216(8):2425-2442. doi: 10.1083/jcb.201612129. Epub 2017 Jun 28. PMID: 28659328
Learn moreFour-Channel Super-Resolution Imaging by 3-D Structured Illumination
Early Commissural Diencephalic Neurons Control Habenular Axon Extension and Targeting
Curr Biol. 2017 Jan 23;27(2):270-278. doi: 10.1016/j.cub.2016.11.038. Epub 2017 Jan 5. PMID: 28065605
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