Z02

Advanced imaging

Funding period: 2017-2029

Project Z02

Project Leaders

Dr. Ulrike Engel

 Project Leader Z02

Dr. Gary Davidson

 Project Leader Z02

Abstract

Advanced imaging of biological processes has become an essential part of research however few laboratories have the required equipment or expertise to incorporate this easily into their research projects. The availability of managed on-site advanced imaging facilities for CRC members to collaborate with closely is what Z02 offers. In addition to a wide variety of advanced fluorescence-based microscopy, we also provide NanoBiT/BRET methodology for the analysis of ligand-receptor interactions, focusing on Wnt-FZD pairing.

Project-related Publications

WNT5a export onto extracellular vesicles studied at single-molecule and single-vesicle resolution

Schubert A, Mongkolsittisilp A, Kobitski A, Schulz M, Voloshanenko O, Schaffrinski M, Winkler N, Neßling M, Richter K, Kranz D, Nienhaus K, Jäger D, Trümper L, Büntzel J, Binder C, Nienhaus GU, Boutros M. FEBS J. 2025 Apr 1.

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Developmental signals control chromosome segregation fidelity during pluripotency and neurogenesis by modulating replicative stress

de Jaime-Soguero A, Hattemer J, Bufe A, Haas A, van den Berg J, van Batenburg V, Das B, di Marco B, Androulaki S, Böhly N, Landry JJM, Schoell B, Rosa VS, Villacorta L, Baskan Y, Trapp M, Benes V, Chabes A, Shahbazi M, Jauch A, Engel U, Patrizi A, Sotillo R, van Oudenaarden A, Bageritz J, Alfonso J, Bastians H, Acebrón SP. Nat Commun. 2024 Aug 28;15(1):7404. doi: 10.1038/s41467-024-51821-9. PMID: 39191776

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Wnt/β-catenin signaling induces axial elasticity patterns of Hydra extracellular matrix

Veschgini M, Suzuki R, Kling S, Petersen HO, Bergheim BG, Abuillan W, Linke P, Kaufmann S, Burghammer M, Engel U, Stein F, Özbek S, Holstein TW, Tanaka M. iScience. 2023 Mar 16;26(4):106416. doi: 10.1016/j.isci.2023.106416. eCollection 2023 Apr 21. PMID: 37009232

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Superresolution microscopy localizes endogenous Dvl2 to Wnt signaling-responsive biomolecular condensates

Schubert A, Voloshanenko O, Ragaller F, Gmach P, Kranz D, Scheeder C, Miersch T, Schulz M, Trümper L, Binder C, Lampe M, Engel U, Boutros M. Proc Natl Acad Sci U S A. 2022 Jul 26;119(30):e2122476119. doi: 10.1073/pnas.2122476119. Epub 2022 Jul 22. PMID: 35867833

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Genetically encodable fluorescent protein markers in advanced optical imaging

Nienhaus K, Nienhaus GU. Methods Appl Fluoresc. 2022 Jun 29. doi: 10.1088/2050-6120/ac7d3f. Online ahead of print.
PMID: 35767981

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Allele-specific endogenous tagging and quantitative analysis of β-catenin in colorectal cancer cells

Ambrosi G, Voloshanenko O, Eckert AF, Kranz D, Nienhaus GU, Boutros M. Elife. 2022 Jan 11;11:e64498. doi: 10.7554/eLife.64498.
PMID: 35014953

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Two plus one is almost three: a fast approximation for multi-view deconvolution

Hüpfel M, Fernández Merino M, Bennemann J, Takamiya M, Rastegar S, Tursch A, Holstein TW, Nienhaus GU. Biomed Opt Express. 2021 Dec 7;13(1):147-158. doi: 10.1364/BOE.443660. eCollection 2022 Jan 1. PMID: 35154860

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Wnt signaling recruits KIF2A to the spindle to ensure chromosome congression and alignment during mitosis

Bufe A, García Del Arco A, Hennecke M, de Jaime-Soguero A, Ostermaier M, Lin YC, Ciprianidis A, Hattemer J, Engel U, Beli P, Bastians H, Acebrón SP. Proc Natl Acad Sci U S A. 2021 Aug 24; 118(34):e2108145118. doi:10.1073/pnas.2108145118. PMID: 34417301

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Measuring ligand-cell surface receptor affinities with axial line-scanning fluorescence correlation spectroscopy

Eckert AF, Gao P, Wesslowski J, Wang X, Rath J, Nienhaus K, Davidson G, Nienhaus GU. Elife. 2020 May 22;9:e55286.

PMID: 32441251

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Wnt/PCP controls spreading of Wnt/β-catenin signals by cytonemes in vertebrates

Mattes B, Dang Y, Greicius G, Kaufmann LT, Prunsche B, Rosenbauer J, Stegmaier J, Mikut R, Özbek S, Nienhaus GU, Schug A, Virshup DM, Scholpp S. Elife. 2018 Jul 31;7. PMID:30060804

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Characterization of spindle pole body duplication reveals a regulatory role for nuclear pore complexes

Rüthnick D, Neuner A, Dietrich F, Kirrmaier D, Engel U, Knop M, Schiebel E. J Cell Biol. 2017 Aug 7;216(8):2425-2442. doi: 10.1083/jcb.201612129. Epub 2017 Jun 28. PMID: 28659328

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Four-Channel Super-Resolution Imaging by 3-D Structured Illumination

Engel U. Methods Mol Biol. 2017;1663:79-94. doi: 10.1007/978-1-4939-7265-4_7.
PMID: 28924660

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Early Commissural Diencephalic Neurons Control Habenular Axon Extension and Targeting

Beretta CA, Dross N, Guglielmi L, Bankhead P, Soulika M, Gutierrez-Triana JA, Paolini A, Poggi L, Falk J, Ryu S, Kapsimali M, Engel U, Carl M. Curr Biol. 2017 Jan 23;27(2):270-278. doi: 10.1016/j.cub.2016.11.038. Epub 2017 Jan 5. PMID: 28065605

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