Advanced genetic screens and genome engineering
Funding period: 2017-2025
Project Leaders
Abstract
The Z03 Central Project provides access to screening approaches including CRISPR-Cas9 and RNAi screening in cell culture models, as well as time- and spatially-resolved CRISPR and RNAi screening in Drosophila. The Z03 project generates cell lines in which Wnt signaling pathway components are endogenously modified by fluorescent proteins using genome editing to perform quantitative analysis using advanced imaging techniques. The platform supports screening procedures in cell culture models and generates a collection of Drosophila CRISPR lines for tissue-specific in vivo studies.
Project-related Publications
The role of Evi/Wntless in exporting Wnt proteins
Blockade of Wnt Secretion Attenuates Myocardial Ischemia-Reperfusion Injury by Modulating the Inflammatory Response
Tissue-Specific CRISPR-Cas9 Screening in Drosophila
Superresolution microscopy localizes endogenous Dvl2 to Wnt signaling-responsive biomolecular condensates
Proc Natl Acad Sci U S A. 2022 Jul 26;119(30):e2122476119. doi: 10.1073/pnas.2122476119. Epub 2022 Jul 22. PMID: 35867833
Learn moreAllele-specific endogenous tagging and quantitative analysis of β-catenin in colorectal cancer cells
EVI/WLS function is regulated by ubiquitination and linked to ER-associated degradation by ERLIN2
Wnt10b GSK3β dependent Wnt/STOP signaling prevents aneuploidy in human somatic cells
Extracellular vesicles and oncogenic signaling
Mol Oncol. 2020 Nov 18. doi: 10.1002/1878-0261.12855. PMID: 33207034
Learn moreMultiplexed conditional genome editing with Cas12a in Drosophila
eGFP-tagged Wnt-3a enables functional analysis of Wnt trafficking and signaling and kinetic assessment of Wnt binding to full-length Frizzled
Wesslowski J, Kozielewicz P, Wang X, Cui H, Schihada H, Kranz D, Karuna M P, Levkin P, Gross JC, Boutros M, Schulte G, Davidson G. J Biol Chem. 2020 May 7. pii: jbc.RA120.012892. PMID:32381507
Learn moregscreend: modelling asymmetric count ratios in CRISPR screens to decrease experiment size and improve phenotype detection
Imkeller K, Ambrosi G, Boutros M, Huber W. Genome Biol. 2020 Mar 2; 21(1):53. PMID: 32122365
Learn moreA Large-Scale Resource for Tissue-Specific CRISPR Mutagenesis in Drosophila
Exocyst-mediated apical Wg secretion activates signaling in the Drosophila wing epithelium
Chaudhary V, Boutros M. PLoS Genet. 2019 Sep 17;15(9):e1008351. PMID: 31527874
Learn moreToward an integrated map of genetic interactions in cancer cells
Mol Syst Biol. 2018 Feb 21;14(2):e7656. doi: 10.15252/msb.20177656. PMID: 29467179
Learn moreβ-catenin-independent regulation of Wnt target genes by RoR2 and ATF2/ATF4 in colon cancer cells
Voloshanenko O, Schwartz U, Kranz D, Rauscher B, Linnebacher M, Augustin I, Boutros M. Sci Rep. 2018 Feb 16;8(1):3178. PMID:29453334
Learn moreERAD-dependent control of the Wnt secretory factor Evi
Glaeser K, Urban M, Fenech E, Voloshanenko O, Kranz D, Lari F, Christianson JC, Boutros M. EMBO J. 2018 Feb 15;37(4). PMID:29378775
Learn moreMapping of Wnt-Frizzled interactions by multiplex CRISPR targeting of receptor gene families
FASEB J. 2017 Nov;31(11):4832-4844. doi: 10.1096/fj.201700144R. Epub 2017 Jul 21. PMID: 28733458
Learn moreSecretion of Wnt ligands requires Evi, a conserved transmembrane protein
Cell. 2006 May 5;125(3):523-33. doi: 10.1016/j.cell.2006.04.009. PMID: 16678096
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