Research Article

Phosphorylation of Ykt6 SNARE Domain Regulates Its Membrane Recruitment and Activity

membrane attachment secretory pathway trafficking Wnt secretion Ykt6 conformational switch

Karuna M P, Witte L, Linnemannstoens K, Choezom D, Danieli-Mackay A, Honemann-Capito M, Gross JC. Biomolecules. 2020 Nov 16;10(11):E1560.

PMID: 33207719

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Research Article

R-spondins are BMP receptor antagonists in Xenopus early embryonic development

BMP signaling embryonic development R-spondin Wnt signaling

Lee H, Seidl C, Sun R, Glinka A, Niehrs C. Nat Commun. 2020 Nov 4;11(1):5570. 

PMID: 33149137

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Research Article

Multiplexed conditional genome editing with Cas12a in Drosophila

Cas12a Cas9 CRISPR Drosophila genome engineering.

Port F, Starostecka M, Boutros M. Proc Natl Acad Sci U S A. 2020 Aug 25:202004655.

PMID: 32843348

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Research Article

The kinesin motor Klp98A mediates apical to basal Wg transport

endosomal trafficking kinesin transport transcytosis Wnt secretion Wnt signaling
Witte L, Linnemannstöns K, Schmidt K, Honemann-Capito M, Grawe F, Wodarz A, Gross JC. Development. 2020 Aug 14;147(15):dev186833. doi: 10.1242/dev.186833.
PMID: 32665246
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Research Article

Ykt6-dependent endosomal recycling is required for Wnt secretion in the Drosophila wing epithelium

endosomal sorting morphogen trafficking Wnt secretion Wnt signaling

Linnemannstöns K, Witte L, Karuna P, Kittel J, Danieli A, Müller D, Nitsch L, Honemann-Capito M, Grawe F, Wodarz A, Gross JC. Development. 2020 Aug 14;147(15):dev185421. PMID: 32611603

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Other

MAPK signaling links the injury response to Wnt-regulated patterning in Hydra regeneration

Tursch, A., M. Lommel, N. Bartsch, and T.W. Holstein. 2020.  bioRxiv, preprint.

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Research Article

Hysteresis-driven pattern formation in reaction-diffusion-ODE systems

modeling patterning

Alexandra Köthe, Anna Marciniak-Czochra  and  Izumi Takagi. American Institute of Mathematical Science. June  2020, 40(6): 3595-3627. doi: 10.3934/dcds.2020170

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Research Article

Measuring ligand-cell surface receptor affinities with axial line-scanning fluorescence correlation spectroscopy

correlation analysis ligand-receptor binding affinity live imaging physics of living systems quantitative fluorescence microscopy

Eckert AF, Gao P, Wesslowski J, Wang X, Rath J, Nienhaus K, Davidson G, Nienhaus GU. Elife. 2020 May 22;9:e55286.

PMID: 32441251

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Research Article

Protease‐resistant streptavidin for interaction proteomics

chemical derivatization ChIP-SICAP contamination proteomics streptavidin

Rafiee MR, Sigismondo G, Kalxdorf M, Förster L, Brügger B, Béthune J, Krijgsveld J. Mol Syst Biol. 2020 May;16(5):e9370.

PMID: 32400114

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Research Article

eGFP-tagged Wnt-3a enables functional analysis of Wnt trafficking and signaling and kinetic assessment of Wnt binding to full-length Frizzled

bioluminescence resonance energy transfer (BRET) Frizzled fusion protein G protein–coupled receptor (GPCR) ligand binding membrane protein NanoBiT NanoBRET trafficking Wnt signaling

Wesslowski J, Kozielewicz P, Wang X, Cui H, Schihada H, Kranz D, Karuna M P, Levkin P, Gross JC, Boutros M, Schulte G, Davidson G. J Biol Chem. 2020 May 7. pii: jbc.RA120.012892.

PMID:32381507

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Research Article

gscreend: modelling asymmetric count ratios in CRISPR screens to decrease experiment size and improve phenotype detection

Bioconductor Biology-based model CRISPR screen Experimental design Generative probabilistic model Genetic perturbation screen gscreend package

Imkeller K, Ambrosi G, Boutros M, Huber W. Genome Biol. 2020 Mar 2; 21(1):53.

PMID: 32122365

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Research Article

A Large-Scale Resource for Tissue-Specific CRISPR Mutagenesis in Drosophila

conditional mutagenesis CRISPR D. melanogaster developmental biology genetic tools genetics genomics large-scale in vivo screens Resource

Port F, Strein C, Stricker M, Rauscher B, Heigwer F, Zhou J, Beyersdörffer C, Frei J, Hess A, Kern K, Lange L, Langner N, Malamud R, Pavlović B, Rädecke K, Schmitt L, Voos L, Valentini E, Boutros M. Elife. 2020 Feb 13;9:e53865.

PMID: 32053108

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